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產(chǎn)品中心您現(xiàn)在的位置:首頁 > 產(chǎn)品展示 > 技術服務 > 巨噬細胞富集服務 > 巨噬細胞富集服務

巨噬細胞富集服務

更新時間:2025-06-06

簡要描述:

服務簡介從異質(zhì)群體(組織/器官)中分離一種或多種細胞類型是現(xiàn)代生物學研究(單細胞測序等)以及常規(guī)臨床診斷和治療的組成部分

服務簡介

從異質(zhì)群體(組織/器官)中分離一種或多種細胞類型是現(xiàn)代生物學研究(單細胞測序等)以及常規(guī)臨床診斷和治療的組成部分。特定細胞的純化對于基礎細胞生物學研究,特定病理學中的細胞計數(shù)和基于細胞的再生療法都至關重要。

“組學"分析異質(zhì)性的生物學組織樣本時,例如肝臟,血液或骨髓,可能導致有偏差甚至錯誤的結果,尤其是在細胞和/或分子的含量相對較低的百分比/數(shù)量。在這種情況下,整個樣本分析可能會稀釋并掩蓋細胞的特征和/或感興趣的分子。因此,具有良好特性和高質(zhì)量純化的細胞群,才能可靠的用于地檢測其特定特征的細微變化,例如基因表達譜,蛋白質(zhì)表達模式和代謝狀態(tài)。

從群體中分何細胞類型的主要原理是利用一種或多種屬于該細胞類型的特性。根據(jù)粘附,形態(tài)(密度/大?。┖涂贵w結合,可以將泛使用的細胞分離和分離技術大致分類。

具體實驗中,選擇的細胞分離方法通常取決于打算將分離的細胞用于什么目的,并且選擇可能需要權衡。例如,如果非常關注細胞的純度(Purity),則可能會選擇高純度的方法,但這可能會降低產(chǎn)率(Yield)

巨噬細胞廣泛分布于機體全身,可因所處部位的不同而有不同的形態(tài)和名稱,而相對于其他組織或者器官,肝臟巨噬細胞的分離(Separation and Isolation)和富集(Enrichment and Purification)對實驗人員和實驗條件有一定的要求,靶點科技專業(yè)的技術團隊可以提供整套實驗解決方案。

技術原理


separation and ioslation overview副本

percoll centrifugation 副本

isolation by facs and macs副本


儀器設備


水浴鍋

顯微鏡

細胞超凈臺

細胞培養(yǎng)箱

FACS分析儀
FACS分析儀
MACS分選套裝

離心機(制冷,水平轉(zhuǎn)子,帶剎車功能)

 技術優(yōu)勢


重復性和穩(wěn)定性好

成本可控

周期短,效率高

  應用領域


巨噬細胞單細胞組學(蛋白質(zhì)組,轉(zhuǎn)錄組,基因組)研究

生理和病理巨噬細胞功能研究

體外細胞功能研究

動物個體間細胞過繼轉(zhuǎn)移研究

服務流程



 結果展示



  參考文獻



  


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靶點科技(北京)有限公司

靶點科技(北京)有限公司

地址:中關村生命科學園北清創(chuàng)意園2-4樓2層

© 2025 版權所有:靶點科技(北京)有限公司  備案號:京ICP備18027329號-2  總訪問量:340021  站點地圖  技術支持:化工儀器網(wǎng)  管理登陸

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